Housekeeping Gene

Summarized by Plex Health
Last Updated: 09 May 2022
evidence based selection of housekeeping genes. "evidence based selection of housekeeping genes.", by de Jonge HJ, Fehrmann RS, de Bont ES, Hofstra RM, Gerbens F, Kamps WA, de Vries EG, van der Zee AG, te Meerman GJ, ter Elst A. pone-0000898-g002: PCR results of 5 novel candidate housekeeping genes.S indicates sample, cDNA of a HL-60 leukemic cell line was used for all primers, B indicates the blanc (H2O) and L indicates the 100 base pair...

This database provides typical RPKM worths for every housekeeping gene records in 14 separate renal tubule sectors in untreated male Sprague-Dawley rats. Rat renal tubule sections were by hand divided under a high-resolution stereomicroscope. After reverse transcription of poly-tailed mRNA transcripts, cDNAs were magnified and sequenced using an Illumina HiSeq 2000 sequencer. FASTQ series were mapped to rat referral genome rn5. For the objectives of this magazine, "housekeeping gene" is defined as a gene whose mRNA has an RPKM value of a minimum of 1 in all 14 sections. Just how to search the data source: Please use FIND function offered by your internet browser to look for a specific gene symbol. Note that "nephron" describes renal tubule segments from S1 Proximal through DCT, whereas "collecting duct" refers to sections from CNT with IMCD.

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