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Levilinea saccharolytica

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Last Updated: 17 December 2020

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General | Latest Info

Levilinea saccharolytica

Scientific classification
Class:Anaerolineae
Domain:Bacteria
Family:Anaerolineaceae
Genus:Levilinea
Order:Anaerolineales
Phylum:Chloroflexi
Species:L. saccharolytica

Valid publication: Yamada T, Sekiguchi Y, Hanada S, Imachi H, Ohashi, Harada H, Kamagata Y. Anaerolinea thermolimosa sp. Nov., Levilinea saccharolytica gen. Nov., Sp. Nov. And Leptolinea tardivitalis gen. Nov., Sp. Nov., Novel filamentous anaerobes, and description of new classes Anaerolineae classis nov. And Caldilineae classis nov. In bacterial Phylum Chloroflexi. Int J Syst Evol Microbiol 2006; 56: 1331-1340. IJSEM list: Euzeby JP. Notification list. Notification that new names and new combinations have appeared in volume 56, part 6 of IJSEM. Int J Syst Evol Microbiol 2006; 56: 2029-2030. Notes: Nomenclatural type of genus Levilinea Yamada et al. 2006. Publication: Yamada T, Sekiguchi Y, Hanada S, Imachi H, Ohashi, Harada H, Kamagata Y. Anaerolinea thermolimosa sp. Nov., Levilinea saccharolytica gen. Nov., Sp. Nov. And Leptolinea tardivitalis gen. Nov., Sp. Nov., Novel filamentous anaerobes, and description of new classes Anaerolineae classis nov. And Caldilineae classis nov. In bacterial Phylum Chloroflexi. Int J Syst Evol Microbiol 2006; 56: 1331-1340. The Phylogenetic position of this species was determined in genome-scale analysis by Nouioui et al. Publication: Nouioui I, Carro L, Garcia-Lopez M, Meier-Kolthoff JP, Woyke T, Kyrpides NC, Pukall R, Klenk HP, Goodfellow M, Goker M. Genome-base Taxonomic Classification of Phylum Actinobacteria. Front Microbiol 2018; 9: 2007. Assign by: Yamada T, Sekiguchi Y, Hanada S, Imachi H, Ohashi, Harada H, Kamagata Y. Anaerolinea thermolimosa sp. Nov., Levilinea saccharolytica gen. Nov., Sp. Nov. And Leptolinea tardivitalis gen. Nov., Sp. Nov., Novel filamentous anaerobes, and description of new classes Anaerolineae classis nov. And Caldilineae classis nov. In bacterial Phylum Chloroflexi. Int J Syst Evol Microbiol 2006; 56: 1331-1340.

* Please keep in mind that all text is machine-generated, we do not bear any responsibility, and you should always get advice from professionals before taking any actions.

* Please keep in mind that all text is machine-generated, we do not bear any responsibility, and you should always get advice from professionals before taking any actions

GENOME ANNOUNCEMENT

Bacterial Genome Sequencing Service Overview:

DNA ExtractionLibrary PreparationSequencingData Analysis
DNA is extracted from bacterial strain. All samples are validated for high yield and purity.Libraries are generated using TruSeq or Nextera protocols and include a unique barcode sequence. All libraries are verified for quality and yield.Sequencing is performed using the Illumina MiSeq. Up to 96 barcoded samples can be pooled into a single run.Sequences can be aligned to reference genomes or assembled into contigs. All raw and processed data is delivered to the customer.

Levilinea saccharolytica KIBI-1 was isolated from sludge granules of mesophilic wastewater reactor. A Closely related strain was detected in the trichlorobenzene-transforming Microbial consortium. L. Saccharolytica was characterized as an obligately anaerobic, nonmotile, filamentous microbe capable of growth on a range of carbohydrates when supplemented with yeast extract. It grows optimally at 37C and pH 7. 0 Genome of Levilinea saccharolytica KIBI-1 was sequence as part of a project to expand the phylogenetic breadth of Chloroflexi Genomes. Genome sequencing was performed at Seqmatic using Illumina MiSeq sequencing platform. SPAdes version 3. 11 was used to assemble Genome. Genome was screened for contaminants based on sequence coverage, GC composition, and BLAST hits of conserve single-copy genes. Genome Annotation was performed using NCBI Prokaryotic Genome Annotation Pipeline. Draft Genome is 4. 30 Mb in size, assembled into 65 contigs. It encode 3 672 genes, 3 173 coding sequences, 1 16S RNA, and 46 tRNAs. It is estimated to be ~99 % complete based on conserved single-copy genes. The described to date have all been classified as strict anaerobes; however, L. Saccharolytica encodes for branch aerobic respiration pathway. It has Complex I, Complex II, Alternative Complex III, and both-family heme-copper oxygen reductase and bd oxidase. It also encodes for two nitrite reduction pathways: NirS nitrite reductase that reduces nitrite into nitric oxide, and NrfA protein that reduces it into ammonia. The presence of aerobic respiration genes in L. Saccharolytica and other recent sequence Anaerolineae suggest that this Chloroflexi class is substantially more physiologically diverse than previously recognize.

* Please keep in mind that all text is machine-generated, we do not bear any responsibility, and you should always get advice from professionals before taking any actions.

Table

ScaffoldsGenome SizeLongest ScaffoldN50Raw reads
10251528103633081279361067272
* Please keep in mind that all text is machine-generated, we do not bear any responsibility, and you should always get advice from professionals before taking any actions

Sources

* Please keep in mind that all text is machine-generated, we do not bear any responsibility, and you should always get advice from professionals before taking any actions

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