Reverse Transcription Polymerase Chain Reaction Test

Summarized by Plex Health
Last Updated: 04 May 2022

A real-time reverse transcription polymerase chain reaction test for the identification of the H7 subtype in North American Avian influenza viruses was first reported in 2002; nevertheless, recent AIV surveillance efforts in wild birds and H7 break outs in fowl demonstrated that the 2002 test did not discover all H7 AIVs present in North and South America. The logical uniqueness of the new assay was defined with an RNA panel made up of 19 H7 viruses from around the world and RNA from all hemagglutinin subtypes other than H16. The 2008 Pan-American H7 test was revealed to execute similarly to the 2002 test with samplings from poultries experimentally subjected to A/Chicken/BritishColumbia/ 314514-2/ 04 H7N3 extremely pathogenic AIV. The 2008 test was able to discover 100% of the H7 AIV isolates recovered from North American wild birds in a 2006-2007 sample set.

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