Serologic Test

The dengue MAC-ELISA is used for the qualitative detection of dengue virus IgM antibodies. The MAC-ELISA is based on recording human IgM antibodies on a microtiter plate making use of anti-human-IgM antibody followed by the addition of dengue virus antigens. As the body immune system deals with the infection, IgM antibodies versus dengue virus are observable starting 4-5 days after start of symptoms and are accurately detectable for approximately 12 weeks. When results are unfavorable on both tests from the severe specimen, a convalescent phase sampling is needed to make a diagnosis of dengue virus infection. IgM antibody discovery is not useful for dengue virus serotype determination.

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