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Total Lipid Reagent Set

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Last Updated: 18 January 2022

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General | Latest Info

Lipid tests used to assess individuals ' risk of heart attack or stroke. As most important of cardiac risk tests, they provide good, clear indication of whether someone is likely to have coronary event caused by blockage of blood vessels or atherosclerosis. Complete Lipid Profile Involves Routine Tests Such As Total Cholesterol, Triglycerides, HDL Cholesterol And LDL Cholesterol To Measure Levels Of Triglycerides And Cholesterol In Blood. In addition, we also offer extended lipid profile and assay emerging risk factors of cardiovascular disease. To discover more about our range of lipid assays, select option from drop-down menu. Alternatively, read on to gain of lipids and relevancy of Randox lipid assays. Cardiovascular disease is number 1 cause of death globally! Cardiovascular disease occur as result of reduced blood flow to heart, brain or body and include coronary heart disease, cerebrovascular disease and peripheral arterial disease. Reduce blood flow may be result of blood clotting or due to build-up of fatty acids in arteries causing them to narrow. This can result heart attack or stroke. Lipid tests are in risk assessment of cardiovascular disease. Lipid tests aim to assess risk of cardiovascular disease by screening for abnormalities of triglyceride and cholesterol levels in blood. Triglycerides and cholesterol are types of fat in blood. Triglycerides, main constituent of body fat, are produced by liver as well as obtained through diet. Cholesterol is fat-like substance found in all cells of body and is produced by liver for use by body; its uses include making hormones, vitamin D, and substances that assist in food digestion. Triglycerides and cholesterol contribute to build-up of fatty deposits, and thus lipid profiling enables preventative measures to be taken to maintain safe levels and reduce chances of cardiovascular disease. If you are worried about cardiovascular health, ask your doctor to assess your lipids profile. Routine tests include Total Cholesterol test assessing overall cholesterol levels, HDL Cholesterol test to measure level of good cholesterol in blood, LDL Cholesterol test to measure level of bad cholesterol in blood, and Triglycerides test assessing triglyceride levels. In addition to routine tests, Randox offers range of extra tests for extended analysis of blood lipids and emerging risk factors. Our extended lipids profile includes measurement of lipoproteins, proteins in blood which enable triglycerides, cholesterol and fats to move to appropriate cells.

* Please keep in mind that all text is machine-generated, we do not bear any responsibility, and you should always get advice from professionals before taking any actions.

* Please keep in mind that all text is machine-generated, we do not bear any responsibility, and you should always get advice from professionals before taking any actions

Product overview

As previously state, rapid and inexpensive methods for estimating lipid content in oleaginous microorganisms are required to screen numerous candidate and identify optimal cultivation conditions. To this end, various systems enabling processing of large numbers of strains in reasonable time are being developed and are discussed below. IR spectroscopy has gained as fast and non-invasive Lipid quantification method that can be applied to small amounts of whole cells for real-time monitoring and screening of cultures. Flow-cytometry and Fourier transform infrared spectroscopy, supported by multivariate analysis, have been proposed as fast and reliable means to monitor and quantify lipids over time in oleaginous yeasts Cryptococcus curvatus, Rhodosporidium toruloides, Lipomyces starkeyi. Microtiter plate cultivation systems combined with FTIR have also been used as high-throughput online platform for screening of oleaginous filamentous fungi and to evaluate efficiency of Lipid extraction process in oleaginous fungi. FTIR analysis various microorganisms and their sample preparation are present in Table 5. FTIR has been apply since 2000 to detect in microalgae. For example, Giordano et al. Study carbon allocation pattern in Chaetoceros muelleri Under nitrogen-limited conditions. They suggest that, under such conditions, carbon was direct towards Lipid rather than protein and chlorophyll synthesis, irrespective of source of nitrogen. Similar study Herad et al. Using synchrotron IR micro-spectroscopy examined distribution of lipids and proteins in microalgae Micrasterias Hardyi when supply of phosphorus and nitrogen was restore. Sigee et al. Study carbon allocation during phosphorus limitation in subspicatus using synchrotron-base FTIR micro-spectroscopy and suggest that Lipid accumulation in this microalgae was more pronounced under cellular phosphorus limitation than extracellular phosphorus starvation. FTIR micro-spectroscopy was also used by other authors to investigate allocation of carbon towards lipids in various microalgal species. Although these studies were on shifting ratio of macromolecules inside cells, exact quantification of lipids inside cellular matrix by FTIR could not be achieved until chemometric correlation was proposed by Laurens and Wolfrum. These authors developed multivariate calibration method that enables quantitative analysis of exogenous spiked neutral and polar lipids in four different microalgae on basis of near-infrared and FTIR spectroscopy. However, correlation between amount of phosphatidylcholine and FTIR signal was sometimes problematic, which might have been due to interference by TAGs whose spectra were similar to that of phosphatidylcholine. Indeed, signal interference remains major challenge of using FTIR to quantify lipids in microbial cells and results should be cross-check with other methods. NMR spectroscopy can be used to determine composition of complex biological samples. It requires minimal sample preparation and is of low as it does not require chromatographic separation or derivatization of sample, plus it can be applied to liquid or solid samples. NMR-active nuclei in Lipid molecules include carbon, hydrogen, oxygen, and phosphorus, with 1 H NMR being most common Lipid profiling of algal samples.

* Please keep in mind that all text is machine-generated, we do not bear any responsibility, and you should always get advice from professionals before taking any actions.

* Please keep in mind that all text is machine-generated, we do not bear any responsibility, and you should always get advice from professionals before taking any actions

Material and methods

IR spectra were recorded by means Varian 2000 FTIR spectrometer equipped with Mercury-Cadmium-Telluride detector and single reflection diamond ATR accessory. Volume of 3 L of sample was placed on top of diamond ATR crystal and film spectra were collected after solvent evaporation. At least three measurements were done. Spectroscopic protein-to-Lipid ratio was determined using protocol described in. Briefly, after PBS background spectral subtraction, relative amount of protein was estimated by integrated intensity protein Amide I band while lipid content was approximated by integrated area of C-H stretching bands from 3020-2800 cm 1 wavenumber region. Conversion of spectroscopic protein-to-Lipid ratio to nominal protein-to-Lipid ratio requires adequate calibration curve. For this purpose, BSA-Brain Total Lipid Extract mixtures in PBS buffer with varying protein-to-Lipid ratios were used.

* Please keep in mind that all text is machine-generated, we do not bear any responsibility, and you should always get advice from professionals before taking any actions.

* Please keep in mind that all text is machine-generated, we do not bear any responsibility, and you should always get advice from professionals before taking any actions

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